ProjectInfluence of glucocorticoids on the extracellular matrix and mechanosensitivity of osteoblasts.
Basic data
Title:
Influence of glucocorticoids on the extracellular matrix and mechanosensitivity of osteoblasts.
Duration:
01/07/2022 to 30/06/2024
Abstract / short description:
In the project applied for here, the influence of glucocorticoids (GCs) on the extracellular matrix (ECM) and the transduction of mechanical stimuli of mesenchymal stromal cells (MSCs) and osteoblasts will be investigated in more detail.
In our own preliminary work for this project, we have detected changes in ECM composition by GCs during osteogenic differentiation of MSCs. We hypothesize that these changes have an impact on the mechanical properties of the cells (elasticity, strength), as well as the transduction of mechanical stimuli e.g. by mechanosensitive ion channels (MSICs), which we will investigate in this project using atomic force microscopy (AFM) as well as calcium flux (Fluo-4) measurements.
Since the stimulation of osteoblasts and osteocytes by mechanical loading is essential for the maintenance of bone stability, disruption of this process by GCs may be a previously ignored cause of glucocorticoid-induced osteoporosis (GIOP).
By studying the mechanical and mechanosensory properties of the ECM in combination with proteomic and transcriptomic analyses, we aim to investigate this relationship and thereby contribute to the development of new therapeutic or preventive approaches.
In our own preliminary work for this project, we have detected changes in ECM composition by GCs during osteogenic differentiation of MSCs. We hypothesize that these changes have an impact on the mechanical properties of the cells (elasticity, strength), as well as the transduction of mechanical stimuli e.g. by mechanosensitive ion channels (MSICs), which we will investigate in this project using atomic force microscopy (AFM) as well as calcium flux (Fluo-4) measurements.
Since the stimulation of osteoblasts and osteocytes by mechanical loading is essential for the maintenance of bone stability, disruption of this process by GCs may be a previously ignored cause of glucocorticoid-induced osteoporosis (GIOP).
By studying the mechanical and mechanosensory properties of the ECM in combination with proteomic and transcriptomic analyses, we aim to investigate this relationship and thereby contribute to the development of new therapeutic or preventive approaches.
Involved staff
Managers
Department of Orthopedic Surgery
Hospitals and clinical institutes, Faculty of Medicine
Hospitals and clinical institutes, Faculty of Medicine
University Centre of Dentistry, Oral Medicine and Maxillofacial Surgery
Center of Dentistry, Oral Medicine and Maxillofacial Surgery (Department), Hospitals and clinical institutes, Faculty of Medicine
Center of Dentistry, Oral Medicine and Maxillofacial Surgery (Department), Hospitals and clinical institutes, Faculty of Medicine
Contact persons
Faculty of Medicine
University of Tübingen
University of Tübingen
Faculty of Medicine
University of Tübingen
University of Tübingen
Local organizational units
Department of Orthopedic Surgery
Hospitals and clinical institutes
Faculty of Medicine
Faculty of Medicine
University Centre of Dentistry, Oral Medicine and Maxillofacial Surgery
Center of Dentistry, Oral Medicine and Maxillofacial Surgery (Department)
Hospitals and clinical institutes, Faculty of Medicine
Hospitals and clinical institutes, Faculty of Medicine