PEST-BIN

Pioneering Strategies Against Bacterial Infections

01/01/2021 to 31/12/2024

Shotgun proteomics based on high resolution and high accuracy Orbitrap mass spectrometry will be used for large-scale analysis of protein expression and post-translational modifications (PTMs). We will develop and apply protocols to provide a comprehensive resource of the proteome and phosphoproteome pathogen strains under various growth conditions. We will use the obtained datasets to reconstruct networks of protein kinases, protein phosphatases and their substrates (with DTU) and to correlate evolutionary age of proteins with their expression and PTMs using genomic phylostratigraphy (with IRB). Finally, we will use genomics data provided by Sahlgrenska University Hospital to re-annotate ORFs. Our results will provide the most extensive overview of the proteome and PTM dynamics for the analysed pathogens to date, with insights into functional and evolutionary aspects of their genomes and signal transduction networks. Our data will inform biological follow-up experiments of other consortium partners (DTU, CNRS) working on molecular characterization of regulatory pathways, as well as antibiotic development at Naicons.