Entwicklung einer innovativen Methode für die tierversuchsfreie Durchführung einer 28-Tage-Toxizitätsstudie mit einer humanen Langzeit Lebergewebekultur

01/01/2020 to 30/06/2022

The aim of the project is to develop an innovative device that allows the cultivation of small pieces of liver tissue in 3 ml tubes. The device should initially be equipped with 24 tubes, with the possibility of expansion up to
48 or 96 tubes. To ensure perfusion, an innovative system consisting of rotation of the tubes, fins on the tube walls and the control of the oxygen partial pressure is to be developed. In addition, in the course of the project it is intended to make it possible to vary the size, shape and stiffness of the tissue pieces and, in particular, to consider liver stiffness (sclerotization), due to its influence on the metabolism. The advantages of the innovative method compared to the state of the art would be: (i) for the first time better assessment of the risk of DILi occurring in new drugs, (ii) for the first time metabolite profile / toxic effects on human liver tissue can be examined over a longer period of time over 28 days, (iii ) Reduction of animal experiments in drug development, (iv) more sustainable use of the very limited available raw material from human liver resections.