ProjectConverging Parkinson’s disease pathways: Identification of unique and novel gene dependencies
Basic data
Title:
Converging Parkinson’s disease pathways: Identification of unique and novel gene dependencies
Duration:
01/05/2022 to 31/03/2026
Abstract / short description:
• Explore whether increased plasma membrane rigidity coupled to altered NT uptake exists in mitochondrial PD models in addition to PINK1 (Miro1 R272Q) and non-mitochondrial PD neurons (aSyn A30P, LRRK2 G2019S).
• Define mechanism: use nanoparticle tracking to link synaptic defects to vesicular transport and interactions at axons and synapse.
• Perform a CRISPR dependency screen on PD patient PINK1 Q126P and gene corrected lines, starting in human neural progenitor cells (NPCs) to identify genes unique to PD and the survival of dopaminergic neurons.
• Modulation of CRISPR dependency screen using the mitochondrial stressor Rotenone and vehicle control.
• Define mechanism: use nanoparticle tracking to link synaptic defects to vesicular transport and interactions at axons and synapse.
• Perform a CRISPR dependency screen on PD patient PINK1 Q126P and gene corrected lines, starting in human neural progenitor cells (NPCs) to identify genes unique to PD and the survival of dopaminergic neurons.
• Modulation of CRISPR dependency screen using the mitochondrial stressor Rotenone and vehicle control.
Involved staff
Managers
University Department of Neurology
Hospitals and clinical institutes, Faculty of Medicine
Hospitals and clinical institutes, Faculty of Medicine
Other staff
University Department of Neurology
Hospitals and clinical institutes, Faculty of Medicine
Hospitals and clinical institutes, Faculty of Medicine
Local organizational units
Hertie Institute for Clinical Brain Research (HIH)
Non-clinical institutes
Faculty of Medicine
Faculty of Medicine
Funders
London, United Kingdom