Project CoV2TE19 – SARS-CoV-2-T-Zell-Epitope für die Entwicklung neuer diagnostischer und therapeutischer Ansätze für die…

Basic data

Acronym:
CoV2TE19
Title:
SARS-CoV-2-T-Zell-Epitope für die Entwicklung neuer diagnostischer und therapeutischer Ansätze für die COVID-19-Krankheit
Duration:
07/04/2020 to 06/11/2021
Abstract / short description:
SARS-CoV-2 pandemic currently poses a serious threat to the world population, calling for the rapid development of effective diagnostic and therapeutic tools. T-cell immunity mediated by CD4+ and CD8+ T cells represents a cornerstone in the control of viral infections. The knowledge of virus-specific T-cell epitopes represented by HLA-presented peptides provides on the one hand a diagnostic tool and on the other hand various therapeutic options including vaccination approaches and the transfer of virus-specific T cells or T-cell receptors, which was already proven to be effective against various viral infections. Thus, we aim within the proposed project to characterize SARS-CoV-2-specific CD4+ and CD8+ T-cell epitopes from all different proteins of the virus covering the most common HLA allotypes. Using IFN ELISpot assays we will perform a high-throughput screening of 120 potential SARS-CoV-2 HLA class I and class II T-cell epitopes predicted by the established algorithms SYFPEITHI and NetMHCpan using PBMCs from volunteer donors that recovered from SARS-CoV-2 infection (SARS-VD). Validated T-cell epitopes will be further used to characterize SARS-CoV-2 immunity in the SARS-VD cohort as well as in a second cohort of healthy volunteers without any prior virus detection. This approach will finally allow to i) gain more detailed knowledge about the interaction of SARS-CoV-2 with the immune system, ii) provide novel diagnostic tools to identify people with SARS-CoV-2 immunity, and most important iii) define possible target structures for the development of virus-specific immunotherapies for the treatment of the COVID-19 disease.

Involved staff

Managers

Faculty of Medicine
University of Tübingen

Contact persons

Immunology
Interfaculty Institute for Cell Biology (IFIZ), Interfaculty Institutes
Immunology
Interfaculty Institute for Cell Biology (IFIZ), Interfaculty Institutes
Faculty of Medicine
University of Tübingen

Local organizational units

Clinical Cooperation Unit (KKE)
Department of Internal Medicine
Hospitals and clinical institutes, Faculty of Medicine

Funders

Bonn, Nordrhein-Westfalen, Germany
Help

will be deleted permanently. This cannot be undone.