Project Characterisation of novel glycoepetide antibiotic biosynthetic pathways

Basic data

Title:
Characterisation of novel glycoepetide antibiotic biosynthetic pathways
Duration:
01/01/2017 to 31/12/2018
Abstract / short description:
The glycopeptide antibiotics (GPAs) are clinically relevant antibiotic natural products. These are complex molecules and their commercial production relies on the use of bacteria to produce these compounds. This project will
examine the mechanism of biosynthesis of two novel examples of the GPAs (ristomycin and kistamycin) by combining the strengths of the Stegmann group for in vivo manipulation of the bacterial producer strains with
the strengths of the Cryle group for in vitro characterisation of the biosynthetic enzymes (using structural biology and biochemical techniques). The complexity of the biosynthesis machinery requires that we understand their
function on a detailed level using in vitro techniques, however the overall pathway remains far too complex to reconstitute yet in vitro: hence the combination of in vitro experiments directly with the ability to examine the
effects of selective mutations in vivo will enable us to rapidly explore not only the biosynthesis of GPAs but also the potential to make new GPAs through redesign of the biosynthetic machinery itself.

Staff

Managers

Interfaculty Institute of Microbiology and Infection Medicine (IMIT)
Interfaculty Institutes

Contact persons

Faculty of Science
University of Tübingen
Interfaculty Institute of Microbiology and Infection Medicine (IMIT)
Interfaculty Institutes

Local organizational units

Interfaculty Institute of Microbiology and Infection Medicine (IMIT)
Interfaculty Institutes
University of Tübingen

Funders

Bonn, Nordrhein-Westfalen, Germany
Help

will be deleted permanently. This cannot be undone.