Project ANR-Immunoglobulin – Statische und dynamische Eigenschaften von Antikörperproteinlösungen - Einfluss von Crowding…

Basic data

Acronym:
ANR-Immunoglobulin
Title:
Statische und dynamische Eigenschaften von Antikörperproteinlösungen - Einfluss von Crowding und Ladungen
Duration:
18/10/2016 to 17/10/2019
Abstract / short description:
The efficient medical dosage of antibodies requires high
concentrations in aqueous solutions. These solutions can in addition contain other molecules, denoted crowding agents, which help to increase the maximum protein concentration or circumvent protein
aggregation. We propose to study both static and dynamic properties of highly concentrated aqueous antibody solutions using small-angle scattering, neutron spectroscopy, and complementary techniques. In
particular, we address the effects of macromolecular crowding and ion-induced charges on the effective protein-protein interactions, phase behavior, and global as well as internal dynamics of antibodies
in solution. We also focus on the dynamic, transient, or static cluster phases in this system depending on the employed physical parameters. The systematic investigation will cover both the effect of
self-crowding by the antibody proteins themselves as well as by external crowding agents such as other proteins (e.g. BSA), polymers (e.g. PEG), and sugars (e.g. trehalose). With view at the peculiar
three-lobed conformation of immunoglobulin antibody proteins, the project will in particular be guided by the role and importance of the protein shape in the determination of the static and dynamic
properties of the protein solutions. Whilst addressing questions related to biophysics and biomedicine, we will employ both experimental and theoretical concepts and methods from soft-matter physics. These methods provide a unique access to molecular length scales and to picosecond to nanosecond time scales of molecular
motions. Our proposed study addresses important questions regarding applied protein science as well as fundamental mechanisms of crowding in solutions of anisotropic particles.
Keywords:
Antikörper-Proteine
Kolloidphysik
Physik der weichen Materie
Protein-Aggregaten
biophysics
Biophysik
Neutronenstreuung

Involved staff

Managers

Faculty of Science
University of Tübingen
Institute of Applied Physics (IAP)
Department of Physics, Faculty of Science

Contact persons

Faculty of Science
University of Tübingen
Institute of Applied Physics (IAP)
Department of Physics, Faculty of Science

Local organizational units

Institute of Applied Physics (IAP)
Department of Physics
Faculty of Science

Funders

Bonn, Nordrhein-Westfalen, Germany
Help

will be deleted permanently. This cannot be undone.