Project Untersuchung der Depolymerisation von Mikrotubuli durch Kinesin-8 Motoren mittels optischer Pinzetten

Basic data

Title:
Untersuchung der Depolymerisation von Mikrotubuli durch Kinesin-8 Motoren mittels optischer Pinzetten
Duration:
01/01/2016 to 31/12/2018
Abstract / short description:
Microtubules are highly dynamic cellular filaments. The end of microtubules alternate between periods of slow growth and rapid shortening known as dynamic instability. An accurate control of this instability is crucial for many intracellular processes like cell division. In vivo and in vitro studies showed that one of the factors responsible for destabilizing microtubules are motor proteins of the kinesin-8 family. Kinesin-8 can cross-link microtubules and depolymerize them in a length-dependent manner by interacting with their ends. Despite several studies addressing the function of the kinesin-8 motor protein, the molecular mechanism of how kinesin-8 depolymerizes microtubules is still unclear. One hypothesis is that kinesin-8 act cooperatively to depolymerize microtubules. The aim of this proposal is to measure depolymerization forces and test the cooperative depolymerization model by resolving the end-binding activity of single kinesin-8 motors using high-resolution optical tweezers as a sensitive position and force transducer. Understanding the mechanics of kinesin-8 end activity will provide important insights for cell division with implications for future cancer research.
Keywords:
Single Molecule Measurement
Motor Protein
Force Spectroscopy

Involved staff

Managers

Department of Biology
Faculty of Science

Contact persons

Center for Plant Molecular Biology (ZMBP)
Department of Biology, Faculty of Science

Local organizational units

Center for Plant Molecular Biology (ZMBP)
Department of Biology
Faculty of Science

Funders

Bonn, Nordrhein-Westfalen, Germany
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