Project Pflanzliche Exekutor Resistenz Proteine - Analyse der Signalwege mit FORWARD und REVERSE GENETICs

Basic data

Title:
Pflanzliche Exekutor Resistenz Proteine - Analyse der Signalwege mit FORWARD und REVERSE GENETICs
Duration:
07/08/2015 to 31/07/2018
Abstract / short description:
Executor R proteins are a novel, structurally diverse class of proteins that trigger plant immune reactions but that mechanistically differ from well-known NLR-type R proteins. We aim to identify pathway components of executor R proteins to get first insights into their mode of function. We will focus on the analysis of Bs3 and Bs4C, two executor R proteins from pepper. A population of pepper mutant plants will serve as a common platform for complementary forward and reverse genetic screens aimed at identifying components required for Bs3/Bs4C-mediated immune reactions. A forward screen shall identify pathway components by phenotypical screening of the pepper mutant population. Subsequently, causal mutations will be identified by mapping-by-sequencing. In the reverse screen, transcriptome-profiling will identify putative pathway components. These will be functionally studied in corresponding pepper mutant plants identified by TILLING. Once we have identified Bs3/Bs4C pathway components we will clarify how they are involved in the given immune reactions. Our project will provide the first insights into how the executor R proteins Bs3 and Bs4C mediate immune reactions and whether or not distinct executor R proteins and NLR-type R proteins share common signaling components.

Staff

Managers

Faculty of Science
University of Tübingen
Center for Plant Molecular Biology (ZMBP)
Department of Biology, Faculty of Science

Local organizational units

Center for Plant Molecular Biology (ZMBP)
Department of Biology
Faculty of Science

Funders

Bonn, Nordrhein-Westfalen, Germany
Help

will be deleted permanently. This cannot be undone.